LOCUS       pLV-[hsa-miR-545]        9160 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7081..7941
                     /label=Amp(R)
     promoter        8142..8682
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5686..5920
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6495..6950)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5992..6122
                     /label=SV40 pA
     misc_feature    6123..6336
                     /label=SV40 ori
     misc_feature    5007..5603
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3625
                     /label=intron
     CDS             3672..4997
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3415
                     /label=hsa-mir-545 precursor
BASE COUNT     2174 a      2363 c      2472 g      2151 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg attgtattgt 
     3121 aattgtctgt gtatatgcct gtctctcttc ctgttatcag ttagactgtg agctccttga 
     3181 gggcaaggaa ggtgtcttcg tctccagagc ccagcctggc acattagtag gcctcagtaa 
     3241 atgtttatta gatgaataaa tgaatgactc atcagcaaac atttattgtg tgcctgctaa 
     3301 agtgagctcc acaggctctg gattgtcagc tgtttcttca aaaagcatgt ggtcagaaat 
     3361 ttgatatttc tgtatatatc ttcctaagag tttctcatga gacaatgcca tttttgctag 
     3421 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt 
     3481 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc 
     3541 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt 
     3601 tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta cccctctaga gtcgagctac 
     3661 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 
     3721 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 
     3781 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 
     3841 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 
     3901 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 
     3961 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 
     4021 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 
     4081 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 
     4141 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 
     4201 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 
     4261 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 
     4321 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg 
     4381 acccaccggt cgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 
     4441 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 
     4501 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 
     4561 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 
     4621 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 
     4681 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 
     4741 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 
     4801 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 
     4861 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 
     4921 gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc 
     4981 gcaagcccgg tgcctgagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga 
     5041 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 
     5101 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 
     5161 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 
     5221 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 
     5281 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 
     5341 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 
     5401 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 
     5461 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 
     5521 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 
     5581 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac 
     5641 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 
     5701 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 
     5761 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 
     5821 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 
     5881 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 
     5941 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 
     6001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     6061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     6121 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     6181 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     6241 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc 
     6301 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg 
     6361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 
     6421 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 
     6481 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     6541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     6601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     6661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
     6721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     6781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
     6841 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     6901 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact 
     6961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     7021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     7081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     7141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     7201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 
     7261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     7321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     7381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     7441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     7501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     7561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     7621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     7681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     7741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     7801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     7861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     7921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     7981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     8041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     8101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     8161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     8221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 
     8281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     8341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     8401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     8461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     8521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     8581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     8641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     8701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     8761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     8821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     8881 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
     8941 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 
     9001 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 
     9061 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc 
     9121 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
